A colorimetric method for the determination of the proteolytic activity of duodenal juice.
نویسندگان
چکیده
The coupling of diazotized aryl amines with proteins in alkaline solution yield chromophoric protein derivatives. Digestion of a solution of such azoproteins with proteolytic enzymes results in the formation of colored components soluble in trichloroacetic acid. The intensity of the color in the trichloroacetic acid filtrate of the digested substrate is a function of the proteolytic activity of the enzyme solution and serves as the basis for the method here described.
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عنوان ژورنال:
- The Journal of biological chemistry
دوره 171 2 شماره
صفحات -
تاریخ انتشار 1947